Identification of miR397a and Its Functional Characterization in Callus Growth and Development by Regulating Its Target in Liriodendron

Callus growth and development, a crucial process in plant propagation, is involved hormonal balance abundant gene regulation. MiRNAs are key regulators the of cell differentiation development. MiR397 was identified as participating growth, response to stress, it regulated by targeting LAC gene. The regulatory function miR397 during callus development not clear Liriodendron. In this study, LhmiR397a its targets were identified, between LhLAC11 shown using qRT-PCR transient expression protoplasts. Furthermore, clarify LhmiR397a-LhLAC11, transgenic calli overexpressing LhMIR397a, LhLAC11, mLhLAC11 separately obtained Agrobacterium-mediated transfer. results showed that LhMIR397a might retard proliferation, while or could promote proliferation. Genes associated with cycle had decreased when overexpressed, increased observed overexpressed. Additionally, overexpressed generate early cotyledons 21 days after induction, somatic embryo induction time short compared other genotypes. This study provided functional characterization regulating target